Journal: Theranostics

Article Title: High OGT activity is essential for MYC-driven proliferation of prostate cancer cells

doi: 10.7150/thno.30834

Figure Lengend Snippet: Identification of transcription factors that bind to O-GlcNAc marked chromatin. A) Distribution of differently modified histone 3 reads within ±2kb of O-GlcNAc peak. B) . The top 3 motifs enriched for the O-GlcNAc ChIP-seq data showing similarity with known previously identified transcription factor motifs from published datasets. C) O-GlcNAc ChIP-seq consensus overlap with MYC ChIP-seq data reported in this study. D) O-GlcNAc ChIP-seq consensus overlap with MYC ChIP-exo dataset reported previously (GSE73994). E) OSMI-2 disrupts interaction between MYC and HCF-1. Immunoprecipitation (IP) of MYC. IgG denotes negative control. MYC was overexpressed by addition of doxycycline using the LNCaP-MYC cell line for 4 hours either in the presence or absence of 40µM OSMI-2. Experiment was repeated four times. F) Knockout of OGT disrupts the interaction between MYC and HCF-1. Experiment was performed in a mouse embryonic fibroblast cell line that has been genetically engineered to enable removal of OGT gene by addition of 0.5µM Tamoxifen (Tam). After two days of DMSO- or Tam-treatments, cell lysates were prepared and used for immunoprecipitation. Data shown is representative of two biological replicates. G) Overlap of O-GlcNAc (this study), MYC (this study) and HCF-1 (ENCSR000ECH) ChIP-seq data.

Article Snippet: Tamoxifen-inducible OGT knockout mouse embryonic fibroblast (MEF) cell line was obtained from Dr. Natasha Zachara at the CardioPEG CoreC4 (NHLBI P01 HL107153) at Johns Hopkins University School of Medicine .

Techniques: Modification, ChIP-sequencing, Immunoprecipitation, Negative Control, Knock-Out

Journal: Polymers

Article Title: Antibacterial Chitosan Nanofiber Thin Films with Bacitracin Zinc Salt

doi: 10.3390/polym13071104

Figure Lengend Snippet: The cell viability of L929 fibroblasts after incubation with the thin films for 24 h (** p < 0.01).

Article Snippet: The cytotoxicity of the films was estimated from the viability of L929 fibroblasts (KAC Co., Ltd., Kyoto, Japan).

Techniques: Incubation

Journal: International Journal of Molecular Sciences

Article Title: Mts1 (S100A4) and Its Peptide Demonstrate Cytotoxic Activity in Complex with Tag7 (PGLYRP1) Peptide

doi: 10.3390/ijms25126633

Figure Lengend Snippet: ( a ) Cytotoxic activity of the 17.1-Mts1 complex (1 nM) on L929, K562, HEK 293T, and HL-60 cells in the presence of an antibody to TNFR1 (1:100, 24 h). ( b ) Cytotoxic activity of Mts1 complexes with peptides 17.1, 17.1A, and 17.1B on L929 cells (24 h). ( c ) Concentration dependence of the cytotoxic activity of the 17.1-Mts1 complex on L929 cells (24 h). n = 5 for each point (* p -value < 0.05).

Article Snippet: Experiments were performed with the L929 mouse fibroblast cell line, which was cultured, respectively, in DMEM (Himedia Laboratories Private Limited, Maharashtra, India) with 2 mM L-glutamine, 10% FCS (Cytiva LivescienceTM, Marlborough, MA, USA), and antibiotics (penicillin and streptomycin) (Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C in an atmosphere containing 5% CO 2 .

Techniques: Activity Assay, Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Mts1 (S100A4) and Its Peptide Demonstrate Cytotoxic Activity in Complex with Tag7 (PGLYRP1) Peptide

doi: 10.3390/ijms25126633

Figure Lengend Snippet: Cytotoxic activity of the 17.1-Mts1 complex on L929 cells after 3 ( a ) and 24 ( c ) hours in the presence of caspase 3 and 8 inhibitors and necrostatin1. n = 5 for each point, (* p -value < 0.05). ( b ) Western blot with antibodies to caspase 3 from cell lysates 1 h after 17.1-Mts1 addition (left) and control cells (right). ( d ) Western blot with antibodies to phospho-MLKL, RIP3, and phospho-RIP3 from cell lysates 1 h after addition of 17.1-Mts1 ( left ) and control cells ( right ).

Article Snippet: Experiments were performed with the L929 mouse fibroblast cell line, which was cultured, respectively, in DMEM (Himedia Laboratories Private Limited, Maharashtra, India) with 2 mM L-glutamine, 10% FCS (Cytiva LivescienceTM, Marlborough, MA, USA), and antibiotics (penicillin and streptomycin) (Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C in an atmosphere containing 5% CO 2 .

Techniques: Activity Assay, Western Blot, Control

Journal: International Journal of Molecular Sciences

Article Title: Mts1 (S100A4) and Its Peptide Demonstrate Cytotoxic Activity in Complex with Tag7 (PGLYRP1) Peptide

doi: 10.3390/ijms25126633

Figure Lengend Snippet: Cytotoxic activity of the 17.1-M7 complex on L929 cells after 3 ( a ) and 20 ( b ) hours in the presence of caspase 3 and 8 inhibitors, necrostatin1, EGTA, calpains and cathepsins inhibitors, and antioxidant ionol. n = 5 for each point, (* p -value < 0.05).

Article Snippet: Experiments were performed with the L929 mouse fibroblast cell line, which was cultured, respectively, in DMEM (Himedia Laboratories Private Limited, Maharashtra, India) with 2 mM L-glutamine, 10% FCS (Cytiva LivescienceTM, Marlborough, MA, USA), and antibiotics (penicillin and streptomycin) (Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C in an atmosphere containing 5% CO 2 .

Techniques: Activity Assay